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Ekaterinburg

Anthrax Deaths in Russia source from Biological Weapons Program New Scientist Jan 98

A MYSTERIOUS outbreak of anthrax in the Soviet Union in 1979 was caused by an accident at a military plant-something Russia has never admitted-say American and Russian scientists. The finding, and the analytical technique that made it possible, will reinforce last week's decision by US President Bill Clinton to work towards an international agreement on the inspection of suspected germ warfare facilities. In April 1979, 68 people and hundreds of animals died of anthrax in and around Sverdlovsk, now known as Ekaterinburg, an industrial city in the Urals. The US claimed that anthrax spores had escaped from a nearby covert germ warfare laboratory, but Soviet authorities insisted that it was a natural infection carried by animals. Now researchers at the Los Alamos National Laboratory in New Mexico, working with doctors in Ekaterinburg, have proved that the outbreak was not natural. The Russians unearthed tissue samples from the victims preserved in formalin, which the Los Alamos team then examined using the polymerase chain reaction (PCR) to "amplify" tiny amounts of DNA (Proceedings of the National Academy of Sciences, vol 95, p 1224). They identified four out of the five known strains of anthrax in the samples. PCR analysis of natural outbreaks of anthrax among animals, however, revealed only one strain per outbreak. Scott Cram, deputy head of life sciences at Los Alamos, says this confirms that the Sverdlovsk outbreak was not natural. Barbara Rosenberg, who chairs the Federation of American Scientists' working group on biological warfare, says: "This is the final nail in the coffin for the Soviet story." In 1992, a team led by Matthew Meselson of Harvard University found that all the Sverdlovsk victims were downwind of a military plant, and in the same year Russia admitted that the Soviet Union had had a biological warfare programmeBut Russian officials denied that this was anything to do with the incident at Sverdlovsk"The real first here," says Cram, "was identifying strains of anthrax in formalin." This preservative destroys large molecules, so DNA in the Sverdlovsk samples was too fragmented to permit analysis with conventional PCR. But the Los Alamos team developed an ultra-sensitive form of PCR which involves repeatedly applying known stretches of anthrax DNA to the unknown sample to "prime" the amplification of sequences of interest. This, says Rosenberg, is the kind of technique that will be needed if countries agree to inspect each other's biological facilities under the 1972 Biological Weapons Convention. Last week, President Clinton announced that the US was finally dropping its opposition to a tough inspections regime, a stance that had blocked the negotiations. Rosenberg says ultra-sensitive PCR would allow inspectors to identify suspicious pathogens from DNA that had been scrambled to protect commercially sensitive sequences. This had been a major concern for the US. Debora MacKenzie

Anthrax

ANNALS OF WARFARE New Yorker 9 Mar 98

THE BIOWEAPONEERS

In the last few years, Russian scientists bave invented the world's deadliest plagues. Have we learned about this too late to stop it?

BY RICHARD PRESTON

KEN ALIBEK is a quiet man fortyseven years old, with youthful looks and an attractive, open face. He tives in a rented condominium in Arlington, Virginia, a five-minute walk from his office at a private consulting firm. Alibek has dark hair and Asian features, and a dimpled scar on his nose, which he got in an accident that was "not heroic," he says, involving a machine in a biowarfare plant. Before he arrived in the United States, in 1992, Ken Alibek was Dr. Kanatjan Alibekov, the first deputy chief of research and production for the Soviet biological-weapons program. He was the top scientist in the program, a sprawling, clandestine enterprise known as Biopreparat, or The System, by the scientists who worked in it. Biopreparat research-and-production facilities were flung all across the Soviet Union. As Dr. Alibekov, Ken Alibek had thirtytwo thousand scientists and staff people working under him. Alibek has a Doctor of Sciences degree in anthrax. It is a kind of superdegree, which he received in 1988, at the age of thirty-seven, for directing the research team that developed the Soviet Union's most powerful weapons-grade anthrax. He did this research as head of the Stepnagorsk bioweapons facility, in what is now Kazakhstan, which was once the largest biowarfare production facility in the world. The Afibekov anthrax became fitfly operational in 1989. It is an amber-gray powder, finer than bath talc, with smooth, creamy particles that tend to fly apart and vanish in the air, becoming invisible and driffing for miles. The Alibekov anthrax is four times more efficient than the standard product. Ken Alibek is part of a diaspora of biologists who came out of Russia foflowing the breakup of the Soviet Union. Government funding for research decreased dramatically, and scientists who were working in the biowarfare program found themselves without jobs. Some of them went looking abroad. A few have come to the United States or Great Britain, but most went elsewhere. "No one knows where they are," Alibek says. One can guess-that they've ended up in Iraq, Syria, Libya, China, Iran, perhaps Israel, perhaps India--but no one really knows, probably not even the Russian' government. No doubt some of these biologists have carried the Alibekov formi4a in their heads, if not master seed strains of the anthrax and samples of the finished product in containers. The Alibekov anthrax may be one of the more common bioweapons in the world today. It seems plausible that Iraqi biologists, for instance, know the Alibekov formula by now. One day, Ken Alibek and I were sitting in a conference room near his office taMng about the anthrax he and his research team had developed. "It's very difficult to say if I felt a sense of excitement over this. It's very difficult to say what I felt like," he said. "It woulddt be true to say that I thought I was doing something wrong..l thought I had done something very important. The anthrax was one of my [outstanding] scientific personal result." I asked him if he'd tell me the formula for his anthrax. "I cadt say this," he answered. "I won't publish it. I'm just curious," I said. "Look, you must understand, this is unbelievably serious. You can't publish this formula," he said. When I assured him I wouldn't, he told me the formula for the Alibekov anthrax. He uttered just one sentence. The Alibekov anthrax is simple, and the formula is somewhat surprisingi not quite what you'd expect. Two unrelated materials are mixed with pure powdered anthrax spores. It took a lot of research and testing to get the trick right, and Afibek must have driven his research group hard and skdmy to arrive at it. "There are many countries that would to know how to do this," he said.

TILL last week, when Ken Alibek was interviewed on "PrimeTime Live," he was known in this country only to a few government officials and inteuigence experts and defense-industry figures. What he told the C.I.A. and other people with national-security clearances was usually classified. Sometimes the information was so secret that even he couldn't look at his reports once they were issued. "The first report I wrote, I only saw it once from across a room. It was sitting on a table. They wouldrft let me go any closer to it," Alibek says, with a tiny smile. What Alibek describes is shocking, even to those who thought they had a pretty good idea of what bioweapons are out there and who has them. But it is particularly timely now that the public's attention has suddenly focussed on the possibility of biological terrorism, which gained a peculiar intensity in late February, when Larry Wayne Harris and Waliam Leavitt, jr., were arrested by the F.B.I. outside Las Vegas with what was thought to be weapons- grade anthrax in the trunk of a car. The repeated news reports-which turned out to be a false - that they were planning a terrorist attack on the New York City subway system clarified what had seemed to be a vague threat hidden in Iraq. Bioterror had come home. I first heard about Ken Alibek in 1995, although at that time none of my contacts would tefl me his name. He was referred to only as No. 2. (Biodefector No. 1 had come out in 1989.) Last fall, when I finally figured out that No. 2 was Atibekov, I called up a source who has connections to British intelligence and told him I thought I knew who No. 2 was. He cut me off. "Don't say a name," he said. "I can't confirm anything. Have you forgotten that we are talking on an open telephone fine?" That source went nowhere, but then 1 had an idea. For several years, I have known a man named William C. Patrick III, who in certain important re spects is the leading American expert on biological weapons. Before 1969, when President Fichard Nixon shut down the American biowarfare program, Bill Patrick was the chief of product devel opment for the United States Army's biological-warfare laboratories at Fort Detr'ck, Maryland. The "products" that Patrick and his research group developed were powdered spores and viruses that were loaded into bombs and sophisticated delivery systems. Patrick was arguably the top bioweaponeer in the United States. He :E and several hundred other scientists and research-staff members lost their jobs when the biowarfare facilities at Fort Derrick were closed down. (Today, to the best of my knowledge, the scientists at the United States Army Medical Research Institute of Infectious Diseases, or USAMRIID, at Fort Detrick don't make offensive bioweapons. They develop vaccines and treatments to defend against them. As far as I can tell, the United States has no bioweapons, and one piece of evidence for this is that government officials today are remarkably ignorant of them.) Bill Patrick, who is now seventy-one years old, is one of only two or three scientists still alive and active in the United States who have a hands-on technical understanding of bioweapons. As he explained to me, "There's a hell of a disconnect between us fossils who know about biological weapons and the younger generation." In 1991, on the eve of the Gulf War, he was summoned to the Pentagon to take part in a discussion of anthrax. Patrick sat in silence while a group of intelligence analysts, young men and women dressed in suits, discussed anthrax in knowledgeable-sounding voices. "I reached the conclusion that these people didn't know what the hell they were talking about," Patrick recalls. He said, "Have any of you fellows actually seen anthrax?" and he reached into his pocket and pulled out a small jar of amberbrown powder, and hocked it across the table. It rattled and bounced toward the analysts. Theyjerked away, some leaping to their feet. The jar contained anthrax simulant, a biopowder that is essentially identical to anthrax except that it doesn't kill. It is used for experiments in which properties other than infectivity are being tested. "I got that through security, by the way," Patrick observed. Later, Bill Patrick was the oldest United Nations weapons inspector in lraq. The Iraqis knew exactly who he was-the former top scientist in the former American bioweapons program. Iraqi intelligence people started calling his hotel room in Baghdad at night, hissing, "You son of bitch, Patrick," and then hanging up. "It was kind of an honor, but it kept me awake," he says. Today, Bifl Patrick is a consultant to many government agencies the C.I.A., the F.B.I., the Defense Intelligence Agency, the City of New York-on the use of biolo 'cal weapons in a terron'st at91 tack. Jerome Hauer, who is the head of Mayor Rudolph Giuliani's Office of Emergency Management-the group that would handle a bioterror event in New York, should one ever happen-said to me once, "Bill Patrick is one of the only guys who can tell us about some of these biological agents. We afl wonder what we re going to do when he decides to Eght up a cigar and go song." Patrick is able to tell emergency planners what win happen if a biological weapon is released in an American city how many people will die, where they'll die, what the deaths will look like. His reports are classified. Bifl Patrick and Ken Alibek were counterparts. They had been two of the top scientists in what had been the best bioweapons programs on the planet. I speculated that Patrick might know Alibek. "Do I know Ken?" Patrick boomed over the telephone. "We're close friends! My wife and I had Ken over for Christmas this year with our family, because we think he's kind of lonely." Then I thought I understood: Patrick must have participated in the long government discussions with Alibek-the debriefing-that would have taken place after his arrival in the United States. No one else in the U.S. government, not a single soul, would have understood so clearly what Alibek was talking about. The two scientists had become friends during the process.

Isolation unit used for ebola and other bioweapons out-breaks

I drove down to Bill Patrick's house, in Maryland, on a misty day in winter, when leafless white-oak trees and poplars lay in a haze across the slopes of Catoctin Mountain. The clouds pulled apart and the sun appeared, gleaming through cirrus like a nickel. Patrick's house is a modern version of a Swiss chalet, with a view of Fort Detrick and rolling countryside. "Come in, young man," Patrick said genially. A small dog was yapping around his feet. Patrick has a gentlemanly manner, a rather blocky face, with hair combed over a bald head, and penetrating greenish eyes. He glanced at the sky and seemed to sniff the air before ushering me into the house. He is exquisitely sensitive to weather. Alibek arrived a short while later, driving a silver BNM. After lunch, we settled down around the kitchen table. Patrick brought out a bottle of Glen' Scotch whiskey, and we poured ourselves a round. It seemed a very Russian thing to do. The whiskey was smoky and golden, and it moved the talk forward. "You know, I'm disappointed the agency didn't do better by you, Ken," Patrick remarked. He turned to me. "They let him sign up for all these credit cards."

Alibek smiled wryly. "This was a problem." The C.I.A. had introduced him to Visa. "I could buy things with the cards, but it didn't seem like money. Then I found out you have to pay for it later." Alibek speaks English with a mild Russian accent that makes his serious manner seem almost gloomy. He often has a cigarette smoldering between his fingertips, but he works out at a health club, and he has broad, firm shoulders. His brown eyes seem sombre, and he wears black wire-rimmed eyeglasses. He favors linen shirts with band collars, and soft wool-piqu6 jackets in dark, muted colors. He has a calm expression, with a downwardglancing gaze, and he looks vaguely Chinese. Ethnically, he is a Kazakh. He was born and raised in Kazakhstan. In Russia, he was twenty-five pounds heavier, really quite stout, but he says that he is a different person now, even physically. I asked Alibek how he feels about living here. "I'm happy I'm not doing the work," he said. He paused. "I'm not one hundred per cent happy. I know how people feel about me in Russia. Some of my scientific colleagues feel I am a betrayer." Alibek keeps his emotions well hidden, perhaps even from himself He does not laugh easily. When he does laugh, he is clearly enjoying himself, but his body is slightly rigid. He quit Biopreparat in 1991, left Russia with his family, and abruptly ended up in the United States. According to Alibek, some of his former colleagues at Biopreparat-which was privatized-sent word through intermediaries that "if you ever come to Russia you can expect some problems." "I've got no desire to go to Russia," Abbek said, shrugging. He recently separated from his wife, although they enjoy a cordial relationship. She lives near him with their two boys, whom he sees almost every day. His oldest child, a daughter, is studying architecture at an Ivy League university. At times, Abbek has suffered from loneeness and a sense of dislocation, and he has had some concerns about how he wiu support his wife and children in the United States. The Alibeks had a privileged fife in Russia, with drivers to take them everywhere and all the money they could use. The United States government paid him consulting fees while he was briefing scientists and officials, but now he is on his own.

ALIBEK was raised in Alma-Ata, then the capital of Kazakhstan. Alma-Ata is in central Asia, not far from the Chinese border, on the medieval silk route. His first language was Kazakh, and he learned Russian at school. He got a medical degree at the military medical institute at Tomsk. His special interest was infectious-disease epidemiology. At some point while he was still in medical school, he was chosen to work for Biopreparat. Since it was a secret system, you didr;t really apply; you were approached and brought in. He rose fast. In 1982, at the age of thirty-one, he became the acting director of the Omutninsk bioweapons production plant, a major facility in the Kirov region of Russia. Eventually, he ended up working in Biopreparat's headquarters, a large building in Moscow the same building where Biopreparat is situated today. In early April of 1988, Ken Afibek received a telephone call in his office in Moscow. It came from his friend and colleague Lev Sandakhchiev, the director of a Biopreparat facility called Vector, a huge, isolated virology-research campus in the larch forests outside Novosibirsk, a city in western Siberia. In the late nineteen-eighties, Vector was devoted largely to the development and production of virus weapons. (Dr. Sandakhchiev denies this.) Dr. Sandakhchiev reported that there had been an accident. He was reluctant to discuss it on the telephone. "Send me the details in a cryptogram," Alibek said. Once a day for the next fourteen days, Alibek received a new cryptogram about the victim of the accident, Dr. Nikolai Ustinov. Dr. Ustinov was forty-four years old. AEbek recalls him as a fair-skinned man with light-brown hair, ethnically a Russian. He had a wife and children. Alibek thought of him as a good guy and a talented scientist, easy to talk with, receptive to new ideas. Ustinov had been doing basic military research on the Marburg virus, studying its potential as a weapon. The long-term goal was to see if it could be loaded into special biological warheads on the MIRV missaes that were aimed at the United States. (A MIRV has multiple warheads, which are directed at different targets.) At the time, the Soviet biological missile warheads were designed to be loaded with strategic /operational smaflpox virus, Black Death, and anthrax. The Marburg virus had potential for weaponization, too. Marburg is a close cousin to the Ebola virus, and is extremely lethal. Dr. Ustinov had been wearing a spacesuit in a Level 4 hot lab, injecting guinea pigs with Marburg virus. He pricked himself in the finger with a needle, and it penetrated two layers of rubber gloves. Nikolai Ustinov exited through an air lock and a chemical decon shower to Level 3, and used an emergency telephone to call his supervisor. The supervisor decided to put Ustinov into a biocontainment hospital, a twenty-bed unit with steel air-lock doors, like the doors of a submarine, where nurses and doctors wearing spacesuits could monitor him. He was not allowed to speak with his wife and children. Ustinov did not seem to be afraid of dying, but, separated from his family, he became deeply depressed. On about the fourth day, Ustinov developed a headache, and his eyes turned red. Tiny hemorrhages were occurring in them. He requested a laboratory notebook, and he began writing a diary in it, every day. He was a scientist, and he was determined to explain how he was dying. What does it feel hke to die of Marburg virus? What are the psychological effects? For a while, he maintained a small hope that he would@t die, but when his skin developed spontaneous bruises he understood what the future held. Dr. Sandakhchiev's cryptograms to Alibek were dry and factual, and didn't include the human details. Alibek would later learn that perhaps twice Ustinov had broken down and wept. Alibek was frantic to get help to Ustinov. He begged the Ministry of Defense for a special immune serum, but bureaucratic delays prevented its arrival in Siberia until it was too late. When Ustinov began to vomit blood and pass bloody black diarrhea, the doctor gave him transfusions, but as they put the blood into him it came out of his mouth and rectum. Ustinov was in prostration. They debated replacing all the blood in his body with fresh new blood-a so-called whole-body transfusion. They were afraid that that might trigger a total flooding hemorrhage, which would kifl him, so they didn't do it.

ALIBEK did not know exactly which strain of Marburg had infected his colleague. It had been obtained by Soviet intelligence somewhere, but the scientists were never told where strains came from. The Marburg virus seems to live in an unknown animal host in East Africa. It has been associated with Kitum Cave, near Mt. Elgon, so the Soviet strain could have been obtained around there, but AEbek suspected that it came from Germany. In 1967, the virus had broken out at a vaccine factory in Marburg, a small city in central Germany, and had killed a number of people who were working with monkeys that were being used to produce vaccine. One of the survivors was a man named Popp, and Ahbek thought that Ustinov was probably dying of the strain that had come from him. I have seen a photograph of a Marburg monkey worker taken shortly before his death, in late summer, 1967. He is a stout man, lying on a hospital bed without a shirt. His mouth is slack, his teeth are covered with blood. He is hemorrhaging from the mouth and nose. The blood has run down his neck and pooled in the hollow of his throat. It looks spidery, because it's unable to clot. He also seems to be leaking blood from his nipples. The final pages of Dr. Nikolai Ustinov's scientific journal are smeared with unclotted blood. His skin developed starlike hemorrhages in the underlayers. Incredibly-the Vector scientists had never seen this-he sweated blood directly from the pores of his skin, and left bloody fingerprints on the pages of his diary. He wept again before he died. Ken Alibek is nearly hypnotic when he speaks of these things in his flat voice. We sat around the kitchen table as if we were old friends sharing a story. A gray light shone through the kitchen window, and I saw the red flash of a cardinal near the Patricks' bird feeder, almost a ticker of blood. The dog noticed a squirrel, and started barking. "Go get him, Billy," Patrick said, rising to let the dog out. Dr. Ustinov died on April 30, 1988. An autopsy was performed in the spacesuit morgue of the biocontainment hospital. If this was indeed the Popp strain of Marburg virus-and who could say?-It was incredibly lethal. It produced effects in the human body that were stunning, terrifying. Alibek says that a pathology team removed Ustinov's liver and his spleen. They sucked a quantity of his destroyed blood,out of a leg vein using large syringes. They froze the blood and the body parts. They kept the Ustinov strain alive and continually replicating in the laboratories at Vector. They named the strain Variant U, after Ustinov, and they teamed how to mass-produce it in simple bioreactors, flasks used for growing viruses. They dried Variant U, and processed it into an inhalable dust. The particles of Variant U were coated to protect them in the air so that they would drift for many miles. In late 1990, Biopreparat researchers tested airborne Variant U on monkeys and other small animals in special explosion-test chambers at the Stepnagorsk plant. Marburg Variant U proved to be extremely potent in airborne form. They found that just one to five microscopic particles of Variant U lodged in the lungs of a monkey were almost guaranteed to make the animal crash, bleed, and die. With normal weapons-grade anthrax, in comparison, it takes about eight thousand spores lodged in the lungs to pretty much guarantee infection and death. Alibek said that by the fall of 1991, just before Boris Yeltsin came to power, Marburg Variant U was on the verge of becoming a strategic/operational biological weapon, ready to be manufactured in large quantities and loaded into warheads on mirvs. These warheads are sinister things. Ten separate cone-shaped warheads, each targeted on a different location, sit atop a missile. Special cooling ' 'de each warhead keep the systems insi virus alive during the heat of re-entry through the earth's atmosphere. "If we can land a cosmonaut to earth alive, we can do the same with a virus," Alibek explained. "We use parachutes." The biowarheads are parachuted over a city, and at a certain altitude they break apart. Out of each warhead bursts a spray of more than a hundred oval bomblets the size of small cantaloupes. The cantaloupes fly out a distance and then split in overlapping patterns, releasing a haze of bioparticles that quickly becomes invisible. Variant U never became part of the Soviets' strategic arsenal, which was stocked with Black Death, Alibekov anthrax, and powdered smallpox. (Never less than twenty tons of weapons-grade dry smallpox was stockpiled in bunkers.) But it seems quite possible that when the Russian biowarfare facilities fell on hard times and biologists began leaving Russia to work in other countries, some of them carried freeze-dried Variant U with them, ready for further experimentation. Variant U started, perhaps, with a monkey worker named Popp, but its end in the human species is yet to be seen.

A GENERATION ago, biological weapons were called germ-warfare weapons. Biological weapons are very different from chemical weapons. A chemical weapon is a poison ttiat kills upon contact vqth the skin. Bioweapons are rru'croorganisms, bacteria or viruses, that invade the body, multiply inside it, and destroy it. Bioweapons can be used as strategic weapons. That is, they are incredibly powerful and dangerous. They can kill huge numbers of people if they are used properly, and their effects are not limited to one place or a small target. Chemical weapons, on the other hand, can be used only tactically. It is virtually impossible to put enough of a chemical in the air in a high enouah concentration to wipe out a large number of people over a large territory And chemicals aren't ahve and can't spread through an infectious process. There are two basic types of biolog'l-,--l weapons, those that are contagious and those that are not. Anthrax is not contagious: people dodt spread it among themselves; you can't catch anthrax from someone who is dying of it. Smallpox is contagious. It spreads rapidly, magnifying itself, causing mortaety and chaos on a large scale. Like any weapon, a biological weapon can be released accidentally, but when a biological accident happens, the consequences can be particularly insidious. I talked about this with Ken Alibek that day in Bill Patrick's kitchen, while we drank whiskey in the soft fight of a winter afternoon. Alibek spoke about how bioweapons have a disturbing tendency to invade nonhuman populations of living creatures-thus finding a new niche in the ecosystems of the earth, apart from the human species. When he was the acting director of the biowarfare facility at Omutninsk, his safety officers discovered that wild rodents living in the woods outside the factory had become chronically infected with the Schu-4 military strain of tularemia-a bacterium that causes a type of pneumonia which was being made in the plant. It was a hot, lethal strain that came from the United States: an American biological weapon that ttle Soviets had managed to obtain during the nineteen-fifties. Now, unexpectedly, the wAd rodents were spreading Schu-4 among themselves in the forests around Omutninsk. The rodents were not the natural host of tularemia, but it had apparently established itself in them as new hosts. People catch tularemia easily from rodents, and it can be fatal. Alibek mounted an investigation and found that a pipe running through a basement area had a small leak and was dripping a suspension of tularemia cells into the ground. The rodents may have come in contact with the contaminated soil in that one spot. The staff tried to sterilize the forest of rodents near the plant. That didn't work, because rodents are impossible to eradicate. "We could not get rid of the rodents. We tried everything," Abbek said. "Nobody knows today, but we can assume that the tularemia is still there in the rodents." Nobody knows if anyone has died of the American-Russian tularemia around the Kirov region. "Could it have spread across Russia in rodents?" I asked. "This I don't know."

OPREPARAT, or The System, was set B up in 1973, just a year after the Soviet Union signed the Biological and Toxin Weapons Convention, an agreement banning the development, use, and stockpiling of biological weapons. The United States, which had ended its offensive-bioweapons program in 1969, also signed the treaty, as did Great Britain. (Some hundred and forty nations have signed the convention by now.) The Soviets continued to believe, however, that the United States had not ended its bioweapons program but simply hidden it away, turning it into a "blacle' weapons program. "The notion that the Americans had g' ven up their biological weapons was thought of as the great American lie," a British intelligence officer recalls. "In fact, most of the Biopreparat scientists had never even heard of the Biological Weapons Convention." Biopreparat consisted of some forty research-and-production facilities. About a dozen of them were enormous. Perhaps half of the employees developed weapons and the other half made medicines. Biopreparat worked both sides of the street: it cured diseases and invented new ones. An island in the Aral Sea, curiously named Rebirth Island, was used for open-air weapons testing. Large numbers of animals, and perhaps some humans, died there. Biopreparat was modelled to some extent on the Manhattan Project, the program that led to the first atomic bomb. Military people administered the program and scientists did the research-and-development work. Somehow, Biopreparat's weapons program remained invisible to the American scientific community. There was a commonly held behef among many American scientists, supported by the strong, even passionate views of a handful of experts in biological weapons, that the Soviet Union was not violating the treaty. This view persisted, despite reports to the contrary from intelligence agencies, which were often viewed as being driven by right-wing ideology. One of the side effects of the closing of the American bioweapons program was that the United States lost its technical understanding of biological weapons. There has long been a general feeling among American scientists-it's hard to say just how widespread it is, but it is definitely there-that biological weapons do@t work. They are said to be uncontroUable, liable to infect their users, or unworkable in any practical sense. A generation ago, leading physicists in this country understood nuclear weapons because they had built them, and they had observed their effects in field tests and in war. The current generation of Amen'can molecular biologists has been spared the agony of having created weapons of mass destruction, but, since these biologists haven't built them, or tested them, they don't know much about their real performance characteristics. Sitting in Bill Patrick's kitchen, I said to Alibek, "There seems to be a common behef among American scientists that biological weapons aren't effective as weapons. You see these views quoted occasionaUy in newspapers and magazines." Ahbek looked disturbed, then annoyed. "You test them to find out. You learn how to make them work" he said to me. "I had a meeting yesterday at a defense agency. They knew absolutely nothing about biological weapons. They want to develop protection against them, but all their expertise is in nuclear weapons. I can say I don't believe that nuclear weapons work. Nuclear weapons destroy everything. Biological weapons are more ... beneficial. They don't destroy buildings, they only destroy vital activity." "Vital activiy. " "People," he said.

THE first defector to emerge from Biopreparat was Vladimir Pasechnik, a microbiologist, who arrived in Great Britain in 1989, just as the Soviet Union was beginning to crumble. (He was No. 1 to Alibek's No. 2.) Pasechnik frightened British intelligence, and later the C.I.A., when he told them that his work as director of the Institute of Ultrapure Biopreparations, in Leningrad, had involved offensive-biowarfare research into Yersiniapestis, a pestilential microbe that causes plague, or Black Death-an airborne contagious bacterial organism that wiped out a third of the population of Europe around the year 1348. Natural plague is curable with antibiotics. After listening to Dr. Pasechnik, the British concluded that the Soviet Union had developed a genetically engineered strain of plague that was resistant to antibiotics. Because the Black Death can travel through the air in a cough from person to person, a strain of multi-drug-resistant Black Death might be able to amplify itself through a human population in ever-mqdening chains of infection, culminating in a biological crown fire in the human species. No nuclear weapon coidd do that. What was the Soviet Union doing developing strategic contagious biological weapons? "I couldn't sleep at night, thinking about what we were doing," Pasechnik told his British handlers. Even though Western intelligence agencies had known that the Russians had a bioweapons program, they had not known what was being developed, and that the United States was a so-called deep target, far enough away so that the Soviet Union wouldn't be contaminated. President George Bush and Prime Minister Margaret Thatcher were briefed on Pasechniles revela tions, and they put direct personal pressure on Mikhail Gorbachev to open up the biowarfare facieties in the U.S.S.R. to a team of outside inspectors. Eventually, he agreed, and a joint British-American weapons-inspection team toured four of the main Biopreparat facie ' ' January, 1991. The inspec ties in tors visited Vector (the virology complex outside Novosibirsk, where Ustinov died) and a giant, high-security facility south of Moscow called the State Research Center for Applied Microbiology at Obolensk, where they found fer menter tanks-forty of them, each two stories tafl. They were main tained at Biosafety Level 4, inside huge ring-shaped biocontainment zones, in a building called Corpus One. The facility was dedicated to research on a variety of bacterial microbes, especially Yersinia pestis. The Level 4 production tanks were obviously intended for making enormous quantities of something deadly, but when the inspectors arrived the tanks were sparkling clean and sterae. As the British and American weapons inspectors toured the Blopreparat facieties, they ran into the same problems that recently faced the United Nations Special Commission inspectors in Iraq. They were met with denials, evasions, and large rooms that had been stripped of equipment and cleaned up. A British inspector said to me, "Th's was clearly the most successful biological-weapons program on earth. These people just sat there and Eed to us, and lied, and Bed." The deal was that after the Americans and the British had peeked at Biopreparat a team of Soviet inspectors was to visit the United States. In December, 1991, Ken Alibek and a number of leading Blopreparat scientists and military people visited USA-MRED, at Fort Detrick, the Army's Dugway Proving Ground, in Utah, and the Army's old bioweaponsproduction facuity in Pine Bluff, Arkansas, which had been abandoned and partly dismantled in 1969. The Russians stumbled around the weeds in Pine Bluff and saw rusting railroad tracks, buadings with their roofs falling in, and nothing that worked. Alibek was pretty wen convinced by the time he got home that the United States did not have a bioweapons program. But when the final report was issued by the inspectors to the government of Boris Yeltsin it stated that they had found plenty of evidence for a program. Alibek refused to participate in the writing of that report, and he decided to quit Biopreparat. "It was a confused situation," he said. "It was at the exact time when the Soviet Union collapsed. I told all these people I diddt agree with their politics." For a few months, he hung on in Moscow, supporting his family by trading-"It was easy to make money in those days, you could trade anything but he found that his telephone was tapped, and that the K.G.B. had set up a so-called gray unit to watch him, a surveillance team stationed near his apartment. He decided to move his famUy to Alma-Ata, in Kazakhstan. What happened next Alibek refuses to talk about. He will not tell me how he got his family to the United States. Once here, he dropped completely out of sight. It is pretty obvious that he was holed up with American intelligence people, discussing his scientific and technical knowledge with them. Several years went by, and Dr. Alibekov morphed into Ken Alibek.

THE most powerful bioweapons are dry powders formed of tiny particles that are designed to lodge in the human lung. The particles are amber or pink. They have a strong tendency to fly apart from one another, so that if you throw them in the air they disperse Eke a crowd leaving Yankee Stadium. As they disperse, they become invisible to the human eye, normally within five seconds after the release. You can't see a bioweapon, you can't smell it, you can't taste it, and you don't know it was there until days later, when you start to cough and bleed, and by that time you may be spreading it around. Bill Patrick holds five patents on special processes for making biodusts that will disperse rapidly in the air and form an invisible sea of particles. His patents are classified. The U.S. government does not want anyone to obtain Patrick's research. The particles of a bioweapon are exceedingly small, about one to five microns in diameter. You could imagine the size this way: around fifty to a hundred bioparticles Ened up in a row would span the thickness of a human hair. The particles are Eght and fluffy, and don't fall to earth. You can imagine motes of dust dancing in a shaft of sunlight. Dust motes are mostly bits of hair and fuzz. They are much larger than weaponized bioparticles. If a dust mote were as thick as a log, then a weaponized bioparticle would resemble a child's marble. The tiny size of a weaponized bioparticle allows it to be sucked into the deepest sacs of the lung, where it sticks to the membrane, and enters the bloodstream, and begins to rephcate. A bioweapon can kill you with just one particle in the lung. If the weapon is contagious in human-to-human transmission, you will kill a lot of other people, too. So much death emergent from one ' le. Given right weather conditions, a particle bioweapon will drift in the air for up to a hundred miles. Sunlight kills a bioweapon. That is, a bioweapon biodegrades in sunlight. It has a "half-life," like nuclear radiation. This is known as the decay time of the bioweapon. Anthrax has a long decay time it has a tough spore. Taemia has a decay time of only a few minutes in sunlight. Therefore, tularemia should always be released at night. For many years during the nineteen-fifties and sixties, Bill Patrick had his doubts that bioweapons work. Those doubts were removed decisively during the summer of 1968, when one of the biggest of a long series of open-air biological tests was conducted over the Pacific Ocean downwind of Johnston Atoll, a thousand miles southwest of Hawaii. There, in reaches of open sea, American strategic tests of bloweapons had been conducted secretly for four years. Until very recently, these tests remained unknown to people without security clearances. "We tested certain real agents, and some of them were lethal," Patrick said. The American strategic tests of bioweapons were as expensive and elaborate as the tests of the first hydrogen bombs at Eniwetok Atoll. They involved enough ships to have made the world's fifthlargest independent navy. The ships were positioned around Johnston Atoll, upwind from a number of barges loaded "qth hundreds of rhesus monkeys. Late one afternoon, Bifl Patrick went out to Johnston Atoll and stood on the beach to watch a test. At sunset, just as the sun touched the horizon, a Marine Phantom et flew in low, heading on a straight line parallel to the beach, and then continued over the horizon. Meanwhile, a single pod under its wings released a weaponized powder. The powder trailed into the air fike a whiff of smoke and disappeared completely. This was visual evidence that the particles were flying away from one another. Patrick's patents worked. The scientists call this a line-source laydown. The jet was disseminating a small amount of biopowder for every mile of flight (the exact amount is stin classified). One can imagine a jet doing a hnesource laydown over Los Angeles, flying from the San Fernando Valley to Long Beach, releasing dust from a single pod under the wing. It would take a few minutes. The jet would appear on radar, but the trail of bioweapon would be invisible. In Iraq, United Nations inspectors found a vi 'deotape of an Iraqi Phantom jet doing a Ene-source laydown over the desert. The technique looked precisely like the American laydowns, even to the Iraqis' use of a Phantom jet. The one difference was that the Iraqi Phantom had no paot: it was a remote-controlled drone. At johnston Atoll, the Ene of particles moved with the wind over the sea, somewhat like a windshield wiper sweeping over glass. Stationed in the path of the particles, at intervals extending many miles away, were the barges filn of monkeys, manned by nervous Navy crews wearing biohazard spacesuits. The hne of bloparticles passed over the barges one by one. Then the monkeys were taken back to Johnston Atoll, and over the next few days half of them died. Half of the monkeys survived, and were fine. Patrick could see, clearly enough, that a jet that did a laydown of a modest amount of military bioweapon over Los Angeles could kifl half the city. It would probably be more efficient at causing human deaths than a ten-megaton hydrogen bomb. "What was the agent you used?" I asked Patrick. "I don't want to tefl you. It may still be classified. The real reason is that a lot of countries would like to know what we used, and not just the Iraqis. When we saw those test results, we knew beyond a doubt that biological weapons are strategic weapons. We were surprised. Even we didn't think they would work that wen." "But the agent you used was curable with antibiotics, right?" I said. "Sure." "So people could be cured-" "Well, think about it. Let's say you hit the city of Frederick, right here. That's a small city, with a population of about fifty thousand. You could cause thirty thousand infections. To treat the infections, you'd need-let me see." He calculated quickly: "Eighty-four grams of antibiotic per person ... that's ... oh, my heavens, you'd need more than two tons of antibiotic, delivered overnight! There isn't that much antibiotic stored anywhere in the United States. Now think about New York City. It doesn't take a mathematician to see that if you hit New York with a biological weapon you are gonna tie things up for a while."

TODAY, Biopreparat is a much smaller organization than it was during the Soviet years, and it is ostensibly dedicated entirely to peaceful research and production. You can buy face cream and vodka made by Blopreparat. Vector, where Variant U was developed, is no longer part of Blopreparat. The Vector laboratories are undergoing an extremely painfi-il and perhaps incomplete conversion to peaceful use, and the Vector scientists are secretive about some of their work. Dr. Frank Malinoski, who was a member of the British-American team that inspected Vector in the early nineteen-nineties, told me that it is now generally believed that the weapons program has been taken over by the Russian Ministry of Defense. "If Biopreparat was once an egg, then the weapons program was the yolk of the " he said. "They've hard-boiled the egg, egg, and taken out the yolk and hidden it." If, in fact, the yolk exists, what can Western governments do about it? After years of avoiding confrontation with the Russians over bioweapons, Amen'can officials are still uncertain how to proceed. Twenty million dollars or so-no one seems sure of the amount-has been budgeted by a hodgepodge of agencies to offer financial support to Russian biologists for peaceful research (so they woift go abroad). The National Academy of Sciences, for example, spent a million and a half dollars on research funding for the Russians this past year. But the agencies are in a quandary, and fear the scandal that would ensue if it turned out that their fiends had been diverted for weapons research. The yolk of the bioweapons program may now be hidden away in military facuities run by the Russian Ministry of Defense, which are off hmits to Americans. The largest of these is a complex near Sergiyev Posad, an old town about thirty miles northeast of Moscow. It's not clear how much real control Boris Yeltsin has over the Russian military. If the Ministry of Defense wanted to have a bioweapons program, could anyone tell it to stop? One prominent American scientist said to me, "AU of our efforts in touchy-feely relationships have certainly engaged the former Biopreparat people, but we've been turned down flat by the military people. No doubt they're hiding something at Sergiyev Posad, but what are they hiding? Is it a weapons program? Or is it a shadow that doesn't mean anything, Eke the shadow on the shade in 'Home Alone'? We just dorft know." MeanwhUe, there is strong suspicion that at some of the more visible laboratories weapons-related genetic engineering is being conducted. Genetic engineering, in mietary terms, is the creation of genetically altered viruses and bacteria in order to enhance their power as weapons. This work can be done by altering an organism's DNA, which is the ribbonlike molecule that contains the organism's genetic code and is found in every cell and in every virus particle. Three months ago, researchers at the Center for Apphed Microbiology at Obolenskthe place south of Moscow where Biopreparat once developed and massproduced hot strains of Black Death for Soviet missiles and weapons systemspublished a paper in the British medical journal Vaccine describing how they'd created a genetically engineered anthrax. The Obolensk anthrax, they reported, was resistant to the standard anthrax vaccine. Ken Alibek thinks that the Russians published information about their research because "they are trying to get some kind of 'legalization'of military genetic engineering" and because they are proud of their work. The Biological Weapons Convention is vague on exactly what constitutes research into an offensive weapon. Alibek said that the Russian biologists are trying to push the envelope of what is permissible. Then, "if someone other than Boris Yeltsin was in power, they could re-create their entire biologicalweapons program quickly." Western biowarfare experts do@t know if the new engineered anthrax is as deadly as normal anthrax, but it may be, and it could fall into the wrong hands, such as Iraq or Iran. The real problem may he in those countries. Genetic-engineering work can be done in a small buuding by a few Ph.D. researchers, using tabletop machines that are available anywhere in the world at no great cost. In high schools in the United States today, students are taught how to do genetic engineering. They learn how to create new variants of (safe) bacteria which are resistant to antibiotics. One genetic-engineering kit for high-school students costs forty-two dollars and is sold through the mail.

A virus that seems particularly amenable to engineering is smallpox. According to Alibek and others, it is possible that smallpox has left Russia for parts unknown, travelling in the pockets of mercenary biologists. "Iran, Iraq, probably Libya, probably Syria, and North Korea could have smallpox," Alibek said. He bases his hst partly on what Russian intelligence told him while he was in the program, for the Russians were very sensitive to other countries'bioweapons programs, and watched carefidly. Bioweapons programs may exist in Israel (which has never signed the bioweapons treaty) and Pakistan. Alibek is convinced that India has a program. He says that when he was in Biopreparat, Russian intelligence showed him evidence that China has a large bioweapons program. The deadliest natural smallpox virus is known as Varl'ola major. Natural smallpox was eradicated from the earth in 1977, when the last human case of it appeared, in Somalia. Since then, the virus has lived only in laboratories. Smallpox is an extremely lethal virus, and it is highly contagious in the air. When a child with chicken pox appears in a school classroom, many or most of the children in the class may go on to catch chicken pox. Smallpox is as contagious as chicken pox. One case of smallpox can give rise to twenty new cases. Each of those cases can start twenty more. In 1970, when a man in ' fected with smallpox appeared in an emergency room in Germany, seventeen cases of smallpox appeared in the hospital on the floors above. Ultimately, the German government vaccinated a hundred thousand people to stop the outbreak. Two years later in Yugoslavia, a man with a severe case of smallpox visited several hospitals before dying in an intensive-care unit. To stop the resulting outbreak, which forced twenty thousand people into isolation, Yugoslav health authorities had to vaccinate virtually the entire population of the country within three weeks. Smallpox can start the biological equivalent of a runaway chain reaction. About a third of the people who get a hot strain of smallpox die of it. The skin puffs up with blisters the size of hazelnuts, especially over the face. A severe case of smallpox can essentially burn the skin off one's body. The smallpox vaccine wears off after ten to twenty years. None of us are immune any longer, unless we've had a recent shot. There are currently seven million usable doses of smallpox vaccine stored in the United States, in one location in Pennsylvania. If an outbreak occurred here, it might be necessary to vaccinate all two hundred and seventy mdlion people in the United States in a matter of weeks. There would be no way to meet such a demand. "Russia has researched the genetic alteration of smallpox," Alibek told me. "In 1990 and 1991, we engineered a smaflpox at Vector. It was found that several areas of the smahpox genome"-the DNA"can be used for the introduction of some foreign genetic material. The first development was smallpox and VEE." VEE, or Venezuelan equine encephalitis, is a brain virus. It causes a severe headache and near-coma, but it is generally not lethal. Alibek said that the researchers spliced VEE into smallpox. The result was a recombinant chimera virus. In ancient Greek myth, the chimera was a monster made from parts of different animals. Recombination means the mixing of genes from different organ"It's called smaUpox-VEE chimera," isms. I Afibek said. It could also be called Veepox. Under a microscope, Alibek said, the Veepox looks like smallpox, but it isn't. According to Alibek, there was one major technical hurdle to clear in the creation of a workable Veepox chimera, and he says that it took the Vector researchers years to solve the problem. They solved it by finding more than one place in the smallpox DNA where you could insert new genes without decreasing smaupox's abiEty to cause disease. Many researchers feel that the smallpox virus does@t cause disease in animals in any way that is useful for understanding its effects on humans. Alibek says that the Russians tested Veepox in monkeys, but he says that he does@t know the results. More recently, Ahbek claims, the Vector researchers may have created a recombinant Ebola-smallpox chimera. One could call it Ebolapox. Ebola virus uses the molecule RNA for its genetic code, whereas smallpox uses DNA. Alibek beheves that the Russian researchers made a DNA copy of the disease-causing parts of Ebola, then grafted them into smallpox. Alibek said he thinks that the Ebolapox virus is stable-that is, that it will replicate successfiffly in a test tube or in animal@which means that, once created, Ebolapox will hve forever in a laboratory, and wifl not uncreate itself. Thus a new form of hfe may have been brought into the world. "The Ebolapox could produce the form of smallpox called blackpox," Alibek says. Blackpox, sometimes known as hemorrhagic smallpox, is the most severe type of smallpox disease. In a blackpox infection, the skin does not develop bhsters. Instead, the skin becomes dark all over. Blood vessels leak, resulting in severe internal hemorrhaging. Blackpox is invariably fatal. "As a weapon, the Ebolapox would give the hemorrhages and high mortality rate of Ebola virus, which would give you a blackpox, plus the very high contagiousness of smaupox," Alibek said. Bill Patrick became exasperated. "Ken! Ken! I think you've got overkill here. What is the point of creating an Ebola smallpox? 1 mean, it would be nice to do this from a scientific point of view, sure. But with old-fashioned natural smallpox you can bring a society to its knees. You don't need any Ebolapox, Ken. Why, you're 'ust gonna kill everybody." "I suspect that this research has been done," Alibek said calmly. Lev Sandakhchiev, the head of Vector, strongly denies this. "ln our center we developed vaccinia-virus recombinants with VEE viruses and some others," he says. Vaccinia is a harmless virus related to smallpox. It is used for making vaccines. "How much do you think it would cost to create genetically engineered sm,aflpox?" I asked Alibek. "This is not expensive." He paused, thinking. 'A few million dollars. This is what it cost us for making the smaupoxVt'E chimera at Vector in 1990 and 1991."

KEN ALIBFK'S statements about the genetic engineering of smallpox are disturbing. I felt a need to hear some perspective from senior scientists who are close to the situation. Dr. Peter jahrhng is the chief scientist at USAMIUID, and he has visited Russia four times in recent months. ("It seems as if all I do these days is visit Russia," he said to me.) He knows the scientists at Vector pretty well. He has listened to Alibek and questioned him carefiifly, and he doesn't believe him about the Ebola-smallpox chimera. "His talk about chimeras of Ebola is sheer fantasy, in my opinion," jahrhng said. "This would be technically formidable. We have seen zero evidence of the Vector scientists doing that. But a smallpox chimera-is it plausible? Yes, it is, and I think that's scary. The truth is, I'm not so worried about governments anymore. I think genetic engineering has been reduced to simple enough principles so that any reasonably equipped group of reasonably good scientists would be able to construct a credible threat using genetic engineering. I don't think anyone could knock out New York City with a genetically engineered bug, but someone might be able to knock out a few people and thereby make an incredible panic." Joshua Lederberg is a member of a working group of scientists at the National Academy of Sciences who advise the government on biological weapons and the potential for bioterrorism. He is a professor at Rockefeller University, in Manhattan, and is considered to be one of the founders of the biotechnology revolution. He received the Nobel Prize for discovering-in 1946, when he was a young man-that bacteria can swap genes with each other. It was apparent to him even back then that people would soon be moving genes around, for evil as well as for good. I found Lederberg in his office, in a modest buuding covered with vines, in a green island of grass and trees on Manhattan's East Side. He is in his seventies, a man of modest size and modest girth, with a trim white beard, glasses, inteIEgent hazel eyes, and careful sentences. Lederberg knows Ahbek and PasechnikHe said to me, "They are offering very important evi 'dence. You have to look carefiluy at what they're saying, but I offer high credibility to their remarks in general." He seemed to be choosing his words. As far as what was going on at Vector, he says that "With smallpox, anything could have happened. Lev Sandakhchiev is one of the world's authon'ties on the smallpox genome. But there are afl kinds of reasons you'd want to introduce modifications into smallpox." He said that you might, for example, alter smallpox in order to make a vaccine. "You have to prove intent to make a weapon," he said. Researchers normally introduce new genes into-the vaccinia virus. Vaccinia doesn't cause major illness in humans, but if you're infected with it you become immune to smallpox. When the new genes are introduced into vaccinia, they tend to make the virus even weaker, even less able to trigger disease. Putting new genes into smallpox presumably might make it weaker, too. Alibek insisted that the Russians have found, places in the genome of smallpox where you can insert new genes, yet the virus remains deadly. 1 said to Lederberg, "If someone is adding genes from Ebola to smallpox virus, and it's making the smallpox more deadly, as Alibek says is happening in Russia, isn't that evidence of intent to make a weapon?" "No," he said firmly. "You can't prove intent by the experiment it self. It's not even clear to me that adding Ebola genes to smallpox would make it more deadly. What trouble, me is that this kind of work is being done in a clandestine way. They are not telling us what is going on. To be doing such potentially evil research without telling us what they are doing is a provocation. To do an experiment of this kind in the United States would be olmost im possible. There would be an exten sive review, and it might wefl not be allowed for safety reasons. The ex periment is extremely dangerous, because things could get out of hand." Lederberg agreed that Russia does have a clandestine biolo 'cal 91 weapons program today, though it's not at all clear how much Vector and Biopreparat have to do with it, since they are independent entities. As for the biological missues once aimed at the U.S., it doesn't surprise him: "You can put anything in a ballistic missile." Lederberg seems to be a man who has looked into the face of evil for a long time and hasn't bhnked. He is part of a group of scientists and government officials who are trying to maintain a dialogue with Russian biologists and bring them into the international community of science. "Our best hope is to have a dialogue with Sandakhchiev," he said quietly. "There is no technical solution to the problem of biological weapons. It needs an ethical, human, and moral solution if it's going to happen at all. Do@t ask me what the odds are for an ethical solution, but there is no other solution." He paused, considering his words. "But would an ethical solution appeal to a sociopath?"

TERRORISM is the uncontrolled part of the equation. A while ago, Richard Butler, who is the head of the United Nations Special Commission weapons-inspection teams in Iraq, remarked to me, "Everyone wonders what kinds of delivery systems Iraq may have for biological weapons, but it seems to me that the best delivery system would be a suitcase left in the Washington subway." Could something like that happen? What would it be like? The truth is that no one really knows, because lethal bioterror on a major scale has not occurred. At one point in my talk with Ken Ahbek in Bill Patrick's kitchen that winter afternoon, we took a break, and the former master bioweaponeers stood on the lawn outsi 'de the house, looking down on the city of Frederick. The view reaches to the Mt. Airy Ridge, a blue hne in the distance. Clouds had covered the sun again. Patrick was squinting east, with a professional need to understand the nuances of wind and cloud. "The wind is ten to twelve mues an hour, gusting a bit." He pointed to smoke coming from a budding in the valley. "See the smoke there? It's drifting up a little, but see how it hangs? We have sort of an inversion today, not a good one. I'd say it's a good day for anthrax or Q_fever." Afibek fit a cigarette and watched the sky. He appraises weather the same way Patrick does. Suddenly Patrick turned on his heel and went into his garage. He returned in a few moments carrying a large mayonnaise jar. He unscrewed the cap. The jar contained a fine, creamy, fluffy powder, with a mottled pink tinge. The pink was the dried blood of chicken embryos, he explained. "This is a simulant for VEE." It was a fake version of the weaponized brain virus. It was sterile, and had no living organisms in it. It was harmless. The VEE virus can be grown in weapons-grade concentration in live chicken embryos. When the embryos are swimming with virus particles, you break open the eggs (you had better be wearing a spacesuit), and you harvest the sick embryos. You freeze-dry them and process them into a powder using one of Patrick's secret methods. He shook the jar under my face. The blood-tinged powder combed the sides of the jar. A tendril of simulated bioweapon reached for my nose. Instinctively, I jerked my head back. Patrick walked across the lawn and stood by an oak tree. Suddenly he extended his arm and heaved the contents of the jar into the air. His simulated brain-virus weapon blasted through the branches of a dogwood tree and took off in the wind, heading straight down a meadow and across the street, booming with celerity toward Frederick. Within seconds, the aerosol cloud had become invisible. But the particles were there, moving with the breeze at a steady ten to twelve mues an hour. Alibek watched, tugging at his cigarette, nonchalant, mildly amused. "Yeah. You won't see the cloud now." "Some of those particles will go eighteen to twenty miles, maybe to the Mt. Airy Ridge," Patrick remarked. The simulated brain virus would arrive in Mt. Airy in less than two hours. He walked back and put his hand on Alibeles shoulder, and smUed. Afibek nodded. "What are you thinking?" I asked Alibek. He pursed his lips and shrugged. "This is not exciting for me." Patrick went on, "Say you wanted to hit Frederick today, Ken, what would you use?" Alibek glanced at the sky, weighing the weather and his options. "I'd use anthrax mixed with smallpox."

Iraq threatens anthrax scare in Britain Mar 98

Nasser Hindawi the key architect of Iraq's germ warfare program was arrested in late February trying to leave the country. Britain said it placed all ports of entry on alert after an intelligence warning of an anthrax smuggling plot. Saddam Hussein made a threat to flood Britain with anthrax toxin hidden in duty-free bottles of alcohol, cosmetics, cigarette lighters, and perfume sprays, according to a report in the Sun.

A False alarm for Anthrax LAS VEGAS 21 Feb 98

This news report proved exaggerated when it was subsequently found that the anthrax was a safe attenuated vaccine, but the implications and ease wit which someone could manufacture biotoxins and in particular anthrax bacillus remain of concern. Anthrax and botulinus are naturally-occuring and can be harnessed from the wild.

A laboratory owner and a scientist who allegedly once proposed spreading bubonic plague on the New York subway were charged in a United States court yesterday with possessing deadly anthrax bacteria. President Bill Clinton, after being briefed by Attorney-General Janet Reno, quickly assured Americans that the situation was under control and praised authorities for acting quickly. New York Mayor Rudolph Giuliani denied his city's subway system was a possible target. A lawyer for one of the two men, biology laboratory owner William Leavitt, said no anthrax attack was planned and all his client wanted was to develop a vaccine against the disease in humans. Anthrax is an animal disease that is deadly when transmitted to humans. Leavitt, aged 40, who owns labs in Nevada and Frankfurt, Germany, appeared with Larry Wayne Harris, 44, a microbiologist, at a brief court hearing in Las Vegas.

Their case was held over until Tuesday while FBI and military experts examined their borrowed Mercedes. Court papers said the car contained vials of anthrax. Harris is on probation after pleading guilty to illegally buying bubonic plague virus through the US mail. The court papers said Leavitt and Harris offered $US20 million ($34.55 million) to a medical researcher for advanced equipment with which to test anthrax and both men showed up at his laboratory with vials of the bacteria in their possession, The researcher was an informant for the FBI and his information led to the arrests. The court papers said the informant had convictions for extortion in the early 1980s and Leavitt's lawyer, Larnond Mills, said he could not be believed. Harris has called himself a lieutenant-colonel in the Aryan Nations, a white supremacist group, and a follower of Christian Identity, an anti-Semitic and anti-black movement. In court papers he is quoted as proposing in 1997 to spread bubonic plague through the New York subway to wreck the economy, surprise the military and kill hundreds of thousands of people, Earlier in the day, FBI officials told reporters they did not know if the two men had anthrax in their car and that the substances found in it were taken to an air force base for testing. The car was sealed in Plastic to prevent the release Of any bacteria.

Both men were seized on Thursday outside a suburban Las Vegas medical clinic after they brought a Portable cooler into the clinic, Defence DePartment spokesman Ken Bacon said the FBI asked for military experts when the material was found.

REUTERS

Biowarfare Sleuths NS 20 Jun 98 24

COUNTRIES violating the international ban on biological weapons could be caught out by a new Internet database that will highlight unusual outbreaks of disease. The 1975 UN Biological Weapons Convention, which bans all military use of biological agents, has more than 150 signatories. Yet intelligence sources in the US estimate that at least a dozen of these countries have covert biowar programmes. The convention is very difficult to pohce, in part because most toxins have peaceful uses. "There isn't any biological weapons material I am aware of that doesn't also have a legitimate use," says Al Zelicoff, an expert on biological warfare programmes at Sandia National Laboratories in New Mexico. The botulism toxin, for example, is used as a muscle relaxant in some medical treatments. Disease outbreaks caused by accidental leaks of the microorganisms used in bioweapons production may provide the best indication of a treaty violation. Epidemiologists can distinguish such mishaps from natural outbreaks if they know enough about the strains involved and the patients' histories. But currently such sleuthing can take years. For example, conclusive evidence that a 1979 anthrax epidemic in Ekaterinburg, Russia, started with a leak from a military factory emerged only this year (This Week, 7 February, p 5).

To speed the detective work, Zelicoff has set up a database on the Net to which doctors will be able to post details of local disease outbreaks. Changes in disease patterns should show up as they happen. Patient surveys included in the doctors' reports will allow epidemiologists to work out the most likely source of an outbreak. To demonstrate the idea using data on a pathogen not linked to bioweapons, three hospitals in New Mexico and one in the formerly secret Russian military research city Chelyabinsk-70 have arranged to post details on cases of hepatitis C. Although some rogue states may ban their doctors from taking part, Zehcoff expects the public health benefits will entice cliniccs in most countries to participate. Most disease outbreaks reported on the database will be unrelated to biological weapons. But these data will help identify disease hot spots as they appear, making outbreaks easier to control. "It will be difficult for any country to resist taking part," Zelicoff predicts. Zelicoff hopes eventually to hand his database over to an international body such as the WHO. "As it begins to expand, he will need WHO's help," agrees David Heymann, who directs the WHO's emerging diseases division in Geneva. The WHO currently maintains a disease reporting network which connects large clinics, mostly in capital cities, by e-mail and telephone. Zelicoff says his system will have greater reach by extending to doctors working in smaller, regional clinics. Epidemiologists say that between 2000 and 10,000 clinics worldwide would need to become involved for the network to be truly effective. Jonathan Knight, San Francisco

BIOARMAGEDDON Debora McKenzia New Scientist 19 Sep 98

IT BEGINS with a threat. A terrorist group declares that unless its demands are met within 48 hours, it will release anthrax over San Francisco. Two days later, a private plane flies across the Bay, spreading an aerosol cloud that shimmers briefly in the sunlight before disappearing. Scenario one: thousands are killed in the panic as 2 million people flee the city Another 1.6 million inhale anthrax spores. Antibiotics are rushed in, but the hospitals are overwhelmed and not everyone receives treatment. Most of the country's limited stock of anthrax vaccine has already been given to soldiers. Emergency crews provide little help as there are only four germ-proof suits in the whole city. More than a million of the Bay Area's 6.5 million residents die. Scenario two: in the two days before the attack, citizens seal their doors and windows with germ-proof tape. They listen to the radio for instructions, their gas masks, drugs and disinfectants ready. Few panic. When sensors around the city confirm that the cloud contains anthrax spores, hospitals receive the appropriate antibiotics and vaccines. Trained emergency teams with germ-proof suits and tents set up in the places where automated weather analyses show the deadly cloud will drift. With advance preparation and rapid response only 100 000 people die. A terrorist who plans to drop anthrax over San Francisco tomorrow can count on causing the murder and mayhem described in the first scenario-at least according to the simulation that generated these scenarios last year. The question facing policy makers now is: how do we move toward the second scenario? That terrorists may someday turn to biological weapons is no longer a matter of debate (see "All fall down", New Scientist, 11 May 1996, p 32). "Terrorism has changed," says Brad Roberts of the US government-backed Institute for Defense Analyses in Virginia. "Traditional terrorists wanted political concessions," he says. "But now, some groups say their main aim is mass casualties. That makes biological weapons appealing." Terrorists would have little trouble getting their hands on the technology. Hearings in South Africa in June revealed that the apartheid government produced terrorist weapons containing anthrax, Salmonella and cholera. Soviet scientists who have prepared weapons-grade anthrax and smallpox are known to have emigrated, possibly to well-funded terrorist groups like the one run by Osama bin Laden in Afghanistan. Even small groups such as the Aum Shinrikyo sect in Japan have been able to cook up vats of Salmonella or botulin, the toxin that causes botulism. With bioweapons so readily available, how can governments protect us from a terrorist armed with anthrax, smallpox or plague? In May, scientists, policy makers and security experts gathered in Stockhohn to discuss how to limit the devastation of a biological attack on civilians. Until now, most biological defence strategies have been geared to protecting soldiers on the battlefield rather than ordinary people in cities. The situations are quite different, and novel technologies are needed for civilian defence. Suppose a commuter stumbled across a time bomb filled with anthrax on an underground railway platform. Until this year, no police force in the world had any way to disarm such a device safely. One novel solution that attracted attention at Stockholm was a tent full of antiseptic foam. Researchers at Irvin Aerospace in Fort Erie, Ontario, have developed a dome-shaped tent made of ultratough Mylar that can be filled with a stiff foamthe exact composition of which is a closely guarded secret-that kills germs and also neutralises chemical weapons. Once covered by the foam-filled tent, the bomb can be safely detonated. But what if germs are already in the air?

The protective suits and masks used by most emergency services don't have seals tight enough to exclude microorganisms, making them useless against biological attacks, says Jack Sawicki, a former fireman who now works for Geomet Technologies near Washington DC. And the lack of standards for gas masks sold to civilians in most countries makes it as likely that a mask will suffocate you as save you, he says. The suits developed for the military do work-their tight joints and zippers keep bugs out-but they are too pricey for the average fire department. Both Geomet and Irvin Aerospace are about to market civilian bio-suits. In the meantime, other companies are designing protective gear that actually kills pathogens. Molecular Geodesics in Cambridge, Massachusetts, for example, is developing a suit made of a tough, sponge-like polymer that traps bacteria and viruses, which are then destroyed by disinfectants incorporated into the fabric.

Stealthy attack

None of this gear will do any good, however, if the emergency services do not know there has been an attack. And an assault may not be obvious. A terrorist might not use a weapon that goes off with a dramatic bang, or even produces an obvious cloud of germs. The first hint of a biological attack may be a sudden cluster of sick people. Even that will be missed unless someone is watching. And few are. In the US, financial cutbacks have crippled programmes to track disease outbreaks, natural or deliberate. Some could be either, such as food poisoning caused by Escherichia coli 0157 or Salmonella. Medical agencies fear that the extra money requested by President Bill Clinton this spring as part of an anti-terrorist initiative will not be enough to create an adequate surveillance network. In Europe, disease surveillance is only beginning to be organised on the continentwide scale needed to track a biological emergency. But in addition to monitoring infected people, Nicholas Staritsyn of the State Research Centre for Applied Microbiology near Moscow says that more effort should be made to find out which bugs live where. For example, a particular variety of anthrax may occur naturally in South Africa, but not in Canada. Having access to such information could help authorities to distinguish between natural outbreaks and deliberate attacks. Even when infected people start fuming up at local hospitals, early diagnosis of their illness might not be easy, says Steve Morse, who heads the US programme on new diagnostic technologies run by the Defense Advanced Research Projects Agency (DARPA). The first symptoms of anthrax, plague and many other potential agents of bioterrorism resemble those of flu: headaches, fevers, aching muscles, coughing. What's more, some of these symptoms might be brought on by panic attacks, which are likely to be widespread among people who have just been told that they are the victims of a biological attack. One answer discussed in Stockholm would be for hospitals to have the type of high-tech detectors being developed to identify airborne pathogens on the battlefield. With a detector at each bedside, doctors could pick out the volatile molecules released by damaged lung membranes at a very early stage of infection and instantly tell whether a patient was a victim of a biological attack, says Mildred Donlon, head of environmental detection research at DARPA. Eventually, DARPA would like to develop a detector that weighs no more than 2 kilograms, can identify as few as two particles of 20 different biological agents in a sample of air, costs less than $5000 and does not give false negatives. Such detectors could be deployed around cities to give early warning of airborne disease. In the meantime, researchers led by Wayne Bryden at Johns Hopkins University in Baltimore are working on revamping the traditional laboratory workhorse, the mass spectrometer, for use in the field or in hospitals. They've reduced this unwieldy piece of equipment to a suitcase-sized machine that can distinguish between, say, Shigella, which causes dysentery, and Salmonella. A shoeboxsized version, says Bryden, could be ready in five years. Other researchers are experimenting with devices that would not seem out of place in an episode of Star Trek: Voyager. Tiny electronic chips that contain living nerve cells may someday warn of the presence of bacterial toxins, many of which are nerve poisons. Like a canary in a coal mine, the neurons on the chip will chatter until something kills them. "Anything that stops it singing is immediate cause for alarm," says Donlon. While the "canary on a chip" could detect a broad range of toxins, other devices are designed to identify specific pathogens. One prototype consists of a fibre-optic tube lined with antibodies coupled to light-emitting molecules. In the presence of plague or anthrax bacteria, or the toxins botulin or ricin, the molecules light up.

Outwitting our defences

Devices based on antibodies are far from foolproof. First, you need to have the correct antibodies-not easy when you consider the vast number of pathogens you'd need to include, and their everchanging repertoire of surface proteins. "And even the right antibodies can identify only what is on the outside of a particle," Donlon points out. Bugs can be encapsulated in gels or biological polymers to foil antibodies, or normally harmless bacteria engineered to carry nasty genes. "I want to know what is on the inside," she says. To do this, DARPA-funded researchers are developing identification techniques based on RNA analysis. Unlike DNA, which is now used to identify unknown organisms, RNA is plentiful inside cells and need not be amplified before identification begins. And messenger RNA molecules reveal not only what a microorganism is, but what toxins it is making. Once the biological agent has been identified, how do you combat it? Vaccinating people before they are exposed is one answer. This is the strategy the military is betting on. Last year, the US military launched a programme to develop vaccines against potential biological weapons. It will create jabs for diseases for which none exist, such as Ebola, and improve existing vaccines-including the 30-yearold anthrax vaccine being given to 2.4 million American soldiers. But vaccines are no cure-all. An attacker need only generate a germ that sports different antigens to those used in a vaccine to render that vaccine ineffective. Plus, as bioterrorists get more sophisticated, they will develop novel, possibly artificial, pathogens against which conventional vaccines will be useless, predicts Morse. To get around these problems, DARPA is looking at ways of developing vaccines quickly enough for them to be created, mass-produced and distributed after an attack. The first step, which many researchers including those in the fastpaced field of genomics are now working on, involves speeding up DNA sequencing so that an unknown pathogen's genes could be detailed in a day. The resulting sequences could then be the basis for developing an instant DNA vaccine. Making the vaccine is only half the problem, however. Soldiers can be ordered to take shots, but immunising the rest of the population is another matter. Civilians are unlikely to volunteer for the dozens of vaccinations that would be necessary to protect them against every conceivable biological threat. An attack would make many change their minds, but in such circumstances there might not be enough to go around. Although Clinton has called for the stockpiling of vaccines, the US has only 5 million doses of smallpox vaccine-not enough to contain a hypothetical attack, says Ken Berry, president of the American Academy of Emergency Physicians. Ken Alibek, the former second-incommand of the Soviet germ warfare programme who revealed earlier this year that the Soviets had weaponised tonnes of smallpox, argues that it is short-sighted to put too much effort into developing vaccines. Instead, says Alibek, who is now at the Batelle Institute in Virginia, researchers should concentrate on ways to treat victims of biological weapons. Today's antibiotics may be useless because germs could be equipped with genes for resistance to all of them. Russian scientists have already created such a strain of anthrax (This Week, 28 February, p 4). For any treatment to be effective amid the potential chaos of a bioterrorist attack, speed will be of the essence. At the Stockholm meeting, researchers reported their efforts to develop drugs that work against a wide variety of infections and so can be used even before definitive diagnosis. Some are trying to take advantage of recently identified similarities in the way many pathogens produce disease to develop broad-spectrum drugs. For example, Ebola, anthrax and plague all kill their victims by inducing a widespread inflammatory reaction siirlilar to toxic shock syndrome. A team in Cincinnati is testing an anti-inflammatory drug that could stop all of them. Another gang of bacteria-including plague, Salmonella, Shigella and Pseudomonas aeruginosa (one of the bacteria that can cause pneumonia and meningitis)-relies on very similar proteins to latch onto human cells and inject toxins. Drugs that block this system might save people from all these germs, Ake Forsberg of the Swedish Defence Research Establishment in UmeS told the meeting. The trouble with all these new antibioweapon gizmos, gadgets and medicines is that it's far from certain that they would be available in time to shield the first major city targeted for a bioterrorist attack. At the moment, the US is one of the few countries taking the bioterrorist threat seriously. Sweden, France and Israel have trained emergency teams and stockpiled gas masks. Other countries do not seem concerned. "The threat of bioterrorism has not seized our European friends," says Mike Moodie of the Chemical and Biological Arms Control Institute, a thinktank near Washington DC. "They feel it's too improbable." Perhaps they should reconsider. Berry, who helped run the doomsday simulations that ravaged San Francisco, says: "Security experts are not asking if a biological attack on a civilian population is going to happen, but when."